Share. The following multi-step binary elution gradient, based on an water as phase A and acetonitrile as phase B, both containing 0. 00FilterMax F5 Multi-Mode Microplate Reader Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm),. , Ltd. The samples were transferred to black 96-well plates and fluorescence was measured at an excitation wavelength of 405 nm and an emission wavelength of 535 nm on the FilterMax™ F5 Multi-Mode Microplate Reader (Molecular Devices) equipped with the SoftMaxPro Microplate Data Acquisition and Analysis 6. BMG Labtech - THERMOstar. Please note it is NOT a Keyspan USA-19 HS USB adaptor that is commonly used by. Fluorescence intensity was measured with Filter Max F5 Multi-Mode Microplate Readers (Molecular Devices) at an excitation wavelength of 535 nm and an emission wavelength of 590 nm. Z’-LYTE® Compatible Microplate Reader Documentation Version No. Fluorescent intensity was measured at excitation (485 nm) and emission (595 nm) with the FilterMax F5 Multi-mode microplate reader (Molecular Devices). 00 SpectraMax M3, M4, M5, and M5e Multi-Mode Document type: User Manual5016208D 8 Chapter1:Introduction TheFilterMax™ F3Multi-ModeMicroplateReaderandtheFilterMax™F5Multi-Mode MicroplateReaderareidealforabroadrangeofapplications. 3 Pages. 00 USD. Molecular Devices’ FilterMax™ F5 Multi-Mode Microplate Reader was tested for compatibility with Life Technologies’ GeneBLAzer® assays. Caspase-3 activity was used as a marker of cell apoptosis and was assessed according to. 7/4/2018 Great Multi-Mode Microplate Reader Chirag Patel Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. FilterMax F3 & F5 Microplate Readers. 7/4/2018 Great Multi-Mode Microplate Reader Chirag Patel Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. An anti-human HER2/neu human IgG3/κ (Huang and Morrison, 2006) was used as isotype (negative) control. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader. #E1910) in a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA). 50/50. 2 Pages. SpectraMax Glo Steady-Luc Reporter Assay Kit. Cytokines Assay. , San Diego, CA, USA). Once the FilterMax F5 has completely powered on, double-click on the Multi- Mode Analysis Software. ” 3. Optical densities (OD) from ELISA assays were measured with the FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA). 8 Pages. Instrument Configuration: Assay Technology Adapta® & After 30 min, the absorbance of the lysates was measured at 405 nm using a FilterMax F5 Multi-Mode microplate reader. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Add. Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm), Emission Filter Slide #1 (Fluorescence: 535. Relative firefly and Renilla luciferase units were measured in a FilterMax F5 Multi-Mode Microplate Reader using the Dual-Luciferase ® Reporter Assay System (Promega) according to the manufacturer’s instructions. Background light emission was measured using the luminescence setting on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Techniques). Devices FilterMax Multi-Mode Microplate Readers. USD ($) USD - United States Dollar. #0200-7030) Filter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. After incubation, each microplate was analyzed for absorbance reading. Manufacturer: Molecular Devices. ScanLater Western Blot Detection System. Fluorescence intensity was determined using a FilterMax F5 multi-mode microplate reader at an excitation wavelength of 485 and an emission wavelength of 530nm. Each kit was used on the same day that its reagents were reconstituted. 3. CloneMatrix. ] Example of document to evaluate if the reader . By category. In . Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The amount of each HTRA1 protein was. DCF fluorescence was measured by using a microplate reader (FilterMax F5) at maximum excitation and with emission spectra of 485 nm and 535 nm, respectively. 713579). Plaque reduction. The absorbance was measured at 450 nm with a FilterMax F3 & F5 multi-mode microplate reader (Molecular Devices, Sunnyvale, CA). The following document is. com B. The ELISA test was performed using the supernatants from the morphological test. The 0. The supernatant was taken and 0. 0´ 105 dsD NA (p g/ wel ) R F U • Gemini™ EM/XPS Fluorescence Microplate Readers A B C Optimal data qualityGeneral Laboratory Instruments. FlexStation 3 Multi-Mode Microplate Reader. FilterMax™ F3 and F5 Multimode Microplate Readers, Molecular Devices Supplier: Molecular Devices. 2 mm) was measured by a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices) every 15 min over 16 h incubation time. $3,000 USD. com A. com Check our website for a current listing SpectraMax i3/i3x Multi-Mode Microplate Reader SpectraMax Paradigm Multi-Mode Microplate Reader MOLECULAR DEVICES The trademarks used herein are the. Selecting your dsDNA assay kits The family of SpectraMax Quant dsDNA Assay Kits consist of three kits each designed for a specific range of DNA concentrations and optimized for use with florescenceThe cells were washed again, and 90 µL of a substrate solution was added to the wells for 15 min. FilterMax F3 & F5 Multi-Mode Microplate Readers: SpectraMax i3x Multi-Mode Microplate Reader: SpectraMax Paradigm Multi-Mode Microplate Detection System: Citations: Figures: Catalog Number: Inquire: FilterMax F3 / FilterMax F5: i3x: Inquire: Price: Description: The SpectraMax® i3 Multi-Mode Detection Platform comes with standard. SpectraMax iD5. PerkinElmer; Revvity; Customer Transformation Hub. Plate Formats:. Page 4 Operator’s Manual Intended Use Statement • The Synergy H1 is a hybrid multi-mode microplate reader. 7°C and the bottom to 36. SpectraMax i3x Multi-Mode Detection Platform Biotek Synergy H1 Operator's Manual Appliskan User Manual FilterMax F3/F5 Multi- Mode Microplate Readers Spectramax® m2/m2 user guide User manual Software version 3. The absorbance of samples at 405 nm (A 405) was determined at room temperature at 20 and/or 60 min after addition of substrate using a FilterMax F5 Multi-Mode Microplate reader (Molecular Devices, San Jose, CA). 4. The 0. Thereafter, the dye liquid was removed, the formazan was solubilized with dimethyl sulfoxide (DMSO) under continuous agitation for 15 min, and the absorbance of each well was read by the means of a microplate reader (FilterMax F5 Multimode Microplate Reader; Molecular Devices) at a wavelength of 570 µm. Many absorbance assays use a chromogenic substrate, which, upon enzymatic conversion to the final product, results in a compound thatThen 200 µl of 10% (v/v) ammonium hydroxide was added to neutralize the acid and absorbance readings were taken at 405 nm with the FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices. This document is provided to customers. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell. SpectraMax Paradigm Multi-Mode Microplate Reader. 012 fmol 0. F3 F5 F3-SC F5-SC F3 F5. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. 3 Pages. Multi-Mode Readers. A microplate consists of small wells wherein separate reactions take place. The quantity of colorimetric product was continuously monitored for 120 min. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. (PMT) operates in photon counting mode, affording increased sensitivity for red dyes and a linear dynamic range over 6 orders of magnitude. 3 software (Molecular Devices). MetaMorph NX. 4 Pages. 3 Pages. Supplier: Molecular Devices. Designed for researchers with a wide variety of needs, the Thermo Scientific Varioskan LUX Multimode Microplate Reader offers features that help save time and reduce common errors in the lab—including automated. 561. 3 Pages. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of. The Evans blue dye was extracted from the ears with 1 ml of formamide overnight at 55°C and measured spectrophotometrically at 620 nm (FilterMax TM F5 Multi-Mode Microplate Reader). At least three independent experiments were performed for each. x phosphorylation of wild-type BY4741 yeast exposed to 1 μg/ml fludioxonil in 1% DMSO or 0. Protein contamination can be measured at 280 nm. Upgradeable modules are also available including western blot, cell imaging, and fast. 3 Pages. Affordability with unmatched assay performance in a multi-mode microplate reader The FilterMax™ F3 and F5 Multi-Mode Microplate Readers provide unmatched. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . 2 mm) was measured by a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices) every 15 min over 16 h incubation time. Page 25: FeaturesThe Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seIMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. Selecting your dsDNA assay kits The family of SpectraMax Quant dsDNA Assay Kits consist of three kits each designed for a specific range of DNA concentrations and optimized for use with florescenceThe SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. FilterMax F5 and F3 Multi‐Mode Microplate ReadersUnpackingand Setup Guide 5016209 B 3 Setting Up the Instrument Setting up theinstrument includes selecting asuitablework area, installing excitation and emission filter slides, connecting thehost computer, and installing thesoftwarethat controls allactions performed by the instrument. 6 reviews. The optical performance is comparable to a top-of-the-line dedicated Molecular Devices SpectraMax 340PC Microplate Spectrophotometer $ 3,500. The amount of the colorimetric product was monitored continuously for 120 min. 4 Pages. An MTT assay was performed with 5 mM BHB in the presence or absence of LPS (1 µg/mL) for 24 h. HTRF-compatible SpectraMax Multi-Mode Readers. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. SoftMax Pro GxP Compliance Software. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumAfter incubation for 6 minutes, the mixture was read at 340 nm using a microplate reader (FilterMax F3/F5 Multi-Mode Microplate Readers, Molecular Devices). 1. Synergy HTX and Synergy LX. Absorbance was detected from the bottom using the FilterMax F5 multimode microplate reader (Molecular Devices, Sunnyvale, CA) at excitation/emission wavelengths of 485 nm/535 nm. 020 fmol 0. 7. State-of-the-art Multi-Mode Microplate Reader. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. 40266 Email: drugdiscoverytech@lifetech. Multi-mode Microplate Readers. Absorbance was measured at 450 nm using a FilterMAx F5 Multi-Mode Microplate Reader and EX2 filter after a 2-hour incubation at 37°C with 10 μL of CCK-8 per 100 μL of media. Signals are detected at two different wavelengths (typically 620 nm and 665 nm), which are used to calculate the. The mixture was then boiled for 10 min. Chemiluminescence measurements were performed using a FilterMax TM F5 Multi-Mode Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA). ScanLater Western Blot Detection System. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument se The method is based on the reaction of malondialdehyde (MDA) with thiobarbituric acid (TBA) under conditions of high temperature and acidity that generates a fluorochrome, which is quantified by fluorimetry (F5 Multi-Mode Microplate Reader), with excitation at 515 nm and emission at 553 nm. Molecular Devices Microplate reader VersaMax $1,495. FilterMax F5 Multi-Mode Microplate Reader The FilterMax F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence,. Page: 1/4NADPH turnover was measured on FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Francisco, USA) at 340 nm over 30 min at 37 °C. 1 at 600 nm as determined by FilterMax F5 multi-mode microplate readers (Molecular Devices). Both the FilterMax F3 and F5 Readers are capable of detecting nucleic acid concentration by fluorogenic means. For WST-1–based assay, 10 μL ready-to-use WST-1 reagent was added to each well and incubated for 3 hours at 37°C, and absorbance at 450 nm was measured. A microplate reader is laboratory machine used for the estimation of biochemical or. Instrument Configuration: Assay Technology Adapta® & FlexStation 3 Multi-Mode Microplate Reader A five-mode microplate reader with integrated fluid transfer Key Features • Five-mode reader with wide range of applications • Flexible liquid transfer enables more assay conditions • User-defined pipetting simplifies assay optimization • Instrument and software validation Adapting to biochemical- and cell-based application requirements while. Then 100 μL of 2 mol L −1 sulphuric acid was added to stop the enzymatic reaction and the absorbance values were read at 450 nm on a FilterMax F5 Multi-Mode Microplate Reader. 40266 Email: drugdiscoverytech@lifetech. F3/F5 Multi-Mode Microplate Readers. 0´ 1 4 4. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 2. Instrument Setup: 1. ”SpectraMax® Paradigm® Multi-Mode Detection Platform. Before unpacking and setting up the FilterMaxTM F3 or FilterMaxTM F5 Microplate Reader, prepare a dry, flat work area that has sufficient space for the instrument, host computer,. The FilterMax F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence, fluorescence polarization (FP), time-resolved fluorescence (TRF), for 6- to 384-well plates. 3 Pages. The values of luminescence are directly proportional to the number of viable cells. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. 8 Pages. F3/F5 Multi-Mode Microplate Readers. 4 Pages. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Neurotransmitter Transporter Uptake Assay Kit. Generally when this occurs, the destination BIG-IP device is unable to execute the tmsh command successfully. Page 137 Using the same plate as was used in step 3, pipette 50 µL/well of buffer • into wells C1–F5 and C10–F12. performs within its specifications. A 1:1 binding ratio Hill model was used to fit the binding interaction data. This instrument has passed specific verification criteria. 003 fmol 0. 00. : 28 Sept 12 Page 2 of 20 Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. . Applications: Absorbance, Cell viability, ELISA, Fluorescence,. , a long-time leader in bioanalytical systems for microplate analysis, today announced the launch of two new multi-mode detection platforms that expand its portfolio of microplate readers, enabling the company to meet all combinations of customer needs now and into the future. The following document is intended to demonstrate setup of this instrument. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Molecular Devices 1311 Orleans Drive Sunnyvale, California 94089 Telephone: +1-408. microplate reader. Transfluor Technology. 100 fmol 384-well (75 µL) 0. The trademarks mentioned herein are the property of Molecular Devices, LLC or their respective owners. Immunoblot analysis was performed with pooled serum (diluted 1:500) separated by SDS-PAGE under non-reducing conditions and transferred onto nitrocellulose membranes (GE Healthcare Life Sciences). Relative firefly and Renilla luciferase units were measured in a FilterMax F5 Multi-Mode Microplate Reader using the Dual-Luciferase ® Reporter Assay System (Promega) according to the manufacturer’s instructions. Delete from my manuals. Align the two slots on the bottle holder with the two pegs on the rail. The hemolysis was calculated with the following formula:Luminescence from the converted ATP was then measured using a FilterMax F5 Multi-mode microplate reader (Molecular Devices). shopping cart 0 items $0. Luminescence (glow) Fluorescence polarization. OMRF research scientist Kumar Subramani reviewed BioTek Synergy H1, stating it is a configurable multi-mode microplate reader with high sensitivity. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumFlexStation 3 Multi-Mode Microplate Reader A five-mode microplate reader with integrated fluid transfer Key Features • Five-mode reader with wide range of applications • Flexible liquid transfer enables more assay conditions • User-defined pipetting simplifies assay optimization • Instrument and software validation Adapting to biochemical- and cell. Approximately 50 µl of these. 5. An MTT assay was performed with 5 mM BHB in the presence or absence of LPS (1 µg/mL) for 24 h. 5 fmol/100 μl fluorescein in Fluorescence Intensity-Bottom Read Mode, 15 amol/100 μl Europium in Time-Resolved Fluorescence Mode, 4 mP at 10nM Fluorescein in. The FilterMax™ F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence, fluorescence polarization (FP), and time-resolved fluorescence (TRF) for 6- to 1536. BMG LABTECH - CLARIOstar Plus . Click on the microplate icon in the upper left corner and then click "New" to open a new data file: In the section "Plate1", click on the Settings icon. Microplate readers, multi-mode, FilterMax™ F3 and F5 Supplier: Molecular Devices Description: The FilterMax™ series of multi-mode microplate readers can be operated as standalone instruments or can be integrated seamlessly with laboratory autom. Figure 2: Images of colonies of commonly used cell types raised in semi-solid culture in CloneMedia. The data were analyzed using Multi-Mode Analysis software (Molecular Devices, Corp. Fluorescence was measured using a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA, USA). Table of Contents. 1 Software Molecular Devices’ FilterMax™ F5 Multi-Mode Microplate Reader was tested for compatibility with Life Technologies’ GeneBLAzer® assays. The 50% cytotoxic concentration (CC 50) of F5-2a was calculated. The plate was incubated for 1 hr at room temperature with shaking. Synergy™ H1 is a flexible monochromator-based multi-mode microplate reader. * Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Molecular Devices for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above. This document is provided to customers. Published Dec 16, 2020 | Updated Dec 16, 2020. The ucs load command creates a backup of. Dave Zeckhausen. 00 USD. The experiment was carried out with two biological replicates and three technical replicates. ” e. CloneMatrix. • DNA/RNA quantitation • Reporter gene assay The FilterMax™ F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence, fluorescence polarization (FP), and time-resolved fluorescence (TRF) for 6- to 1536-well plates. Click “Eject Plate Carrier. FilterMax F5 Multi-Mode Microplate Reader. Multi-Mode microplate readers offer flexibility and ease of use over a broad range of applications. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. SpectraMax iD5. SpectraMax Gemini XPS/EM Microplate Readers. In addition, the iD5 reader can measure TRF and FP, and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter gene detection with syringe and Western blot detection. HEK-Blue-hTLR4 and HEK-Blue-hTLR2 cells were incubated with. They provide absorbance, fluorescence, glow luminescence, and other read modes for. All Synergy HT models support the reading of 6-, 12-, 24-, 48-, 96-, and 384-well microplates with standard 128 x 86 mm geometry. Each condition was performed in. 4 Pages. URL of this page: HTML Link: Bookmark this page. DMSO was used to dissolve the compounds and was included in the microplate at 0. Lonza Luminometers Instrument MycoAlert™ Assay MycoAlert™ PLUS Assay Other Bioassays* Notes Lonza Lucetta™ Luminometer + + Cuvette Tailor -made MycoAlert™ Mode Luminometers from Other SuppliersTriplicate reactions were measured every 2. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. After 2–4 h incubation at 37 °C, the medium was removed and 100 μl dimethyl sulfoxide (DMSO) was added to each well to dissolve the formazan. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . MetaFluor Software. 4 Pages. com A. Perkin Elmer Wallac Victor2 1420 Multilabel Counter $1,500. c. After initial incubation, the lysates were incubated with the caspase-1 substrate Ac-YVAD-pNA at 37 °C. MetaFluor Software. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader $7,900. A blank sample was prepared by mixing. 561. The Labsystems Nepheloskan Ascent Microplate Reader is designed to run with 96-well microplates, for use on applications like immunoprecipitation reactions as well as solubility tests of pharmaceutical components. S. 561. 4 Pages. Select the protocol “Concentration_pico_green_v3. Time-resolved Fluorescence. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Detection modalities are shown in Table 1-1. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Trinean Dropsense 96 Polychromatic. In addition, the FilterMax F5 Reader is capable of measuring. DHE fluorescence was recorded on a Filtermax F5 multi-mode microplate reader. Caspase-3/7 activity, an indicator of apoptosis, was measured by a luminescence assay using caspase-Glo. CloneMatrix. FilterMax F5 and F3 Multi‐Mode Microplate ReadersUnpackingand Setup Guide 5016209 B 3 Setting Up the Instrument Setting up theinstrument includes selecting asuitablework area, installing excitation and emission filter slides, connecting thehost computer, and installing thesoftwarethat controls allactions performed by the instrument. Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. PHA content was calculated based on the equation (fluorescent intensity/OD 600 × 0. Plate Formats:. Seoul, South Korea. Installation Qualification (IQ) FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and. Cell viability was measured in percentages (%) as compared to untreated cells, which were assumed to be 100% viable. The multi-mode microplate reader provides flexibility and ease of use for a variety of applications Market Highlights Microplate Readers Market is predicted to project a notable CAGR of 4. The hemolysis was calculated with the following formula: Hemolysis (%) = (A sample − A PBS)/(A Triton − A PBS. Safety Information. Transfluor Technology. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® Europium• FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. The absorbance of the samples was determined using a FilterMax F3&F5 Multi-Mode Microplate Reader (Molecular Devices). Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm), Emission Filter Slide #1 (Fluorescence: 535. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. 3 Pages. The next day, precultures were diluted to an optical density of 0. The standard curve presented a linear phase from 1. The OD 595 of the culture in the plate (light path length: ∼6. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among. ABS Test results. FilterMax F3 Multi-Mode Microplate Reader Includes Excitation Filter Slide #3 (Absorbance: 405 nm, 450 nm, 492 nm, 620 nm; Fluorescence: 360 nm, 485 nm), Emission Filter Slide #3 (Fluorescence: 465 nm, 535 nm), Multimode Analysis Software, Power Cable, RS-232 Serial Cable, User’s Manuals FilterMax F5 Multi-Mode Microplate ReaderThe upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. Sign In Upload. 3 Pages. MetaMorph NX. FilterMax® F3 & F5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell viability, ELISA, Fluorescence,. Instrumentation and Quality Control. & FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. 2. Each measurement was repeated at least 3 times, and IC 50 values were calculated by non-linear regression in GraphPad Prism 9. 4. The following document is intended to demonstrate setup of this instrument. MICROPLATE READERS Multi-Mode Readers. M3/M4/M5/M5e Multi-Mode Microplate Readers + + Molecular Devices SpectraMax. 2 Temperature and cooling rate control. Page 38 | Getting Started Overview The Cytation 5 is a hybrid multi-mode microplate reader. The instrument performs an initialization procedure that moves the optics and the microplate drawer to home positions. SpectraMax iD3 and iD5 Multi-Mode Microplate Readers;. Place plate in the GloMax® Instrument and read. 5% (v/v) to avoid toxicity. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. After incubation for 2 h at 37 °C, the mixture was centrifuged at 2200 rpm for 10 min to obtain the supernatant. 003 fmol 0. After the absorbance was measured at 450 nm on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA), the concentrations of the samples were determined by. Multi-mode Microplate Readers. (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. , Sunnyvale, CA, USA). The results are expressed as cell viabilities (%) based on the control condition. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . HERM LB 500 with NaI detector Radio HPLC Detector. The OD 600 was plotted against time using Graphpad Prism 8. HEK293T cells were harvested 48 h post-transfection in Passive Lysis Buffer (Promega). The user must evaluate this instrument and PC-based software in conjunction with their specific assay(s). After 30 min, absorbance of the lysates at 405 nm was measured on a microplate reader (FilterMax F5 Multi-Mode microplate reader). Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Molecular Devices Filtermax F5 Multi-mode Microplate Reader BioLogic VMP3 Potentiostat Chassis w/9 VMP3-01 Boards potentiostat SYRINGE/VALVE CHAMBER FOR RQF INSTRUMENT quench flow analysis KIN-TEK RAPID QUENCH FLOW INSTRUMENT KIN-TEK RQF-3 VALVE/SYRINGE CHAMBER Biorad Laboratory Real Time PCR System& FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. Neurotransmitter Transporter Uptake Assay Kit. . & FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. GenePix 4300/4400 Microarray Scanner. Absorbance was measured at 485 nm with FilterMax F5 Multi-Mode microplate readers and the percentage of viable cells was calculated in relation to cells cultured in pure medium. Page 2 FilterMax F5 and F3 Multi‐Mode Microplate Readers Unpacking and Setup Guide 7. com 8. Each measurement was repeated at least 3 times, and IC 50 values were calculated by non-linear regression in GraphPad Prism 9. Cells were harvested for luciferase assay 48 h after transfection. Your applications, your modes, your choice. MetaMorph NX. The absorbance was measured at 450 nm using a FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Corp. Different parameters (for instance, the incubation time of bacteriophage-MBs and S. Instrument Configuration: Assay Technology Adapta® & FilterMax F5 Multi-Mode Microplate Reader. 9). 00 USD. 3. 2 Pages. 6 to 25 ng of prokaryotic recombinant protein reaching a plateau in concentrations above 25 ng. SpectraMax Glo Steady-Luc Reporter Assay Kit. Recommended Optics P/N Filter information 6590-0048 FilterMax™ Fluorescence Excitation Filter 405 nm (bandwidth 30 nm) FilterMax F3 Multi-Mode Microplate Reader Includes Excitation Filter Slide #3 (Absorbance: 405 nm, 450 nm, 492 nm, 620 nm; Fluorescence: 360 nm, 485 nm), Emission Filter Slide #3 (Fluorescence: 465 nm, 535 nm), Multimode Analysis Software, Power Cable, RS-232 Serial Cable, User’s Manuals FilterMax F5 Multi-Mode Microplate Reader Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. Please click on the links below to view the Microplate Reader Comparison Tables. BMG Labtech - FLUOSTAR OMEGA. In the case of cocultures, donor and recipient. Time-resolved Fluorescence. Background light emission was measured using the luminescence setting on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Techniques). The performance characteristics of the data reduction software have not been established with any laboratory diagnostic assay. I. Run a variety of luminometric research applications with the Thermo Scientific Luminoskan microplate luminometer, a compact and robust instrument offering versatile plate formats, fast reading speeds and up to three dispensers. Measurements of Caspase-3/7 Activity. 3 Pages. 05); b Evaluation of the ATP levels of T. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. 3D cell proliferation assays were conducted using high concentrations to optimize conditions for cell lines in 20, 100 or• FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. 5 μg/ml) in the dark for 1 h. Internal controls were. Transfluor Technology. After the absorbance was measured at 450 nm on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA), the concentrations of the samples were determined by extrapolation from a standard curve made in a four-parameter nonlinear regression program in Softmax Pro software (Molecular Devices). SpectraMax iD5. Ammonia and NO 2 Ϫ / NO 3 Ϫ analyses were conducted according to established protocols (37, 38), using a FilterMax F5 multimode microplate reader (Molecular Devices, Canada). The medium was then removed and the cells washed once with 200 μl of ice-cold phosphate buffered saline (PBS). #F5) Methods Standard curves were generated following the assay protocols provided with each kit. Guiberteau MD, in Essentials of Nuclear Medicine Imaging (Sixth Edition), 2012 Photon Transducers. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment. The fluorescence was determined using a fluorimetric microplate reader (FilterMax F5 Multi-Mode Microplate Reader) at 540 and 595 nm for excitation and emission, respectively. Absorbance detection in the UV and visible range (230 nm to 650 nm) Fluorescence intensity; top read and bottom read. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumThe SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. book Page 1 Tuesday, September 7, 2010 6:14 PM. shopping cart 0 items $0. Supernatant (25 μL), or standard, was added and mixed. i3x, SpectraMax M4 or M5, and FilterMax F5 Multi-Mode Microplate Readers. Close Search. FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide Ordering Parts and Accessories To obtain parts and accessories, contact Molecular Devices Customer Service or your authorized service representative.